Investigations of Platelet Function in Whole Blood with BAPA as Anticoagulant.
Transfus Med Hemother
; 37(5): 284-288, 2010.
Article
em En
| MEDLINE
| ID: mdl-21113251
ABSTRACT
BACKGROUND:
Citrate anticoagulation of blood results in non-physiologically low calcium concentration and rapid deterioration of platelet viability. Benzylsulfonyl-D-Arg-Pro-4-amidinobenzylamide (BAPA) as anticoagulant maintains the physiological calcium level and seems to retain platelet function (PF) over a time period of at least 24 h. We evaluated PF in BAPA-anticoagulated peripheral whole blood (WB) between 0.5 and 30 h after blood collection.METHODS:
In WB from 21 healthy volunteers (15 women, 6 men, age range 19-57 years) platelet aggregation (PA) was determined by impedance method and ATP release by luminometry. Platelet response was tested by ADP (10 and 20 µmol/l) and collagen (1 and 2 µg/ml) between 0.5 and 30 h after blood collection.RESULTS:
Parameters of ADP-induced PA showed stable values until 6.5 h after blood collection followed by a significant decline. PA in response to collagen was stable up to 25 h of storage. ATP release induced by collagen displayed a continuous, significant decrease over time.CONCLUSION:
Preservation of platelet response in BAPA-anticoagulated blood depends on the applied agonist showing that collagen-induced PA is more stable compared to ADP known as a weak agonist in WB.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Transfus Med Hemother
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Alemanha