Using multiplex-staining to study changes in the maize leaf phosphoproteome in response to mechanical wounding.
Phytochemistry
; 72(10): 1285-92, 2011 Jul.
Article
em En
| MEDLINE
| ID: mdl-21334701
ABSTRACT
Mechanical wounding of 2-week-old maize (Zea mays L.) leaves, one of the first steps in both pathogen infection and herbivore attack, stimulates metabolism and activates signal transduction pathways dedicated to defense and recovery. The signaling pathways include reversible protein phosphorylation which can modulate protein activities, and transmit signals within cellular pathways and networks. We have used multiplex-staining of high-resolution 2D gels for protein (Sypro Ruby) and phosphorylation (Pro-Q Diamond) as a strategy for quantifying changes in the stoichiometry of phosphorylation after wounding for 270 protein spots. Rigorous statistical analysis of the time-index data allowed us to accept patterns of change in 125 of the spots as non-random, and these patterns were assigned to five clusters. A reliable identity was assigned to 21 selected proteins, most of which have been previously described as phospho-proteins. The results suggest that analysis of protein spots from high-resolution 2D gels by multiplex-staining for protein plus phosphorylation is a strategy that can be broadly useful for study of how the phospho-proteome responds to abiotic stress.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
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Coloração e Rotulagem
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Estresse Fisiológico
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Folhas de Planta
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Zea mays
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Proteoma
Idioma:
En
Revista:
Phytochemistry
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Polônia