Revisiting the central dogma one molecule at a time.
Cell
; 144(4): 480-97, 2011 Feb 18.
Article
em En
| MEDLINE
| ID: mdl-21335233
ABSTRACT
The faithful relay and timely expression of genetic information depend on specialized molecular machines, many of which function as nucleic acid translocases. The emergence over the last decade of single-molecule fluorescence detection and manipulation techniques with nm and Å resolution and their application to the study of nucleic acid translocases are painting an increasingly sharp picture of the inner workings of these machines, the dynamics and coordination of their moving parts, their thermodynamic efficiency, and the nature of their transient intermediates. Here we present an overview of the main results arrived at by the application of single-molecule methods to the study of the main machines of the central dogma.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácidos Nucleicos
/
Enzimas
Idioma:
En
Revista:
Cell
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos