Analysis of multiple growth regulatory proteins using dissociable staining antibody arrays on solid tumor biopsy specimens.
Fetal Pediatr Pathol
; 30(3): 177-88, 2011.
Article
em En
| MEDLINE
| ID: mdl-21355677
ABSTRACT
Growth of tumor cells is often a function of deregulated growth factor receptors and their corresponding intracellular signalling molecules. The dissociable antibody staining arrays have the versatility to rapidly identify the expression, activation, and localization of such molecules and pathways in biopsy specimens. This report describes a protocol to quantify the activity of a panel of signalling molecules in Wilms tumor biopsy specimens and surrounding nonmalignant renal cells. We propose that this technique can be used to rapidly identify multiple markers and may aid in the study of aberrant growth regulatory mechanisms and potential targets for therapeutics from pathologic specimens.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tumor de Wilms
/
Peptídeos e Proteínas de Sinalização Intercelular
/
Análise Serial de Tecidos
/
Neoplasias Renais
/
Proteínas de Neoplasias
Tipo de estudo:
Evaluation_studies
/
Guideline
/
Prognostic_studies
Limite:
Child
/
Humans
Idioma:
En
Revista:
Fetal Pediatr Pathol
Assunto da revista:
PATOLOGIA
/
PEDIATRIA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Canadá