[Breathing activity of suspension culture of cells of Polyscias filicifolia Bailey, Stephania glabra (Roxb.) Miers, and Dioscorea deltoidea Wall].
Prikl Biokhim Mikrobiol
; 47(1): 95-101, 2011.
Article
em Ru
| MEDLINE
| ID: mdl-21442926
ABSTRACT
Peculiarities of breathing of cultures of cells producing biologically active compounds (isoprenoids and alkaloids) were investigated in order to optimize productivity of culture growth and biosynthesis. It had been revealed that studied cultures of cells of Dioscorea deltoidea Wall (producer of furistanol glycosides), Stephania glabra (Roxb.) Miers (producer of stepharin alkaloid) and Polyscias filicifolia Bailey (complex of biologically active agents) differ both in joint breathing activity and in ratio between cytochrome and cyanide-resistant breathing, while changes of rate of total oxygen consumption and activity of alternative oxidase during growth were found to be individual for every investigated culture. Maximum rate of oxygen consumption for cells of D. deltoidea and S. glabra was marked in the period preceding active synthesis of secondary metabolites (lag phase for D. deltoidea and exponential phase for S. glabra). The revealed trends can be used for further monitoring and regulation of growth and biosynthesis of secondary metabolites in producing cell cultures during deep cultivation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Consumo de Oxigênio
/
Araliaceae
/
Dioscorea
/
Stephania
Idioma:
Ru
Revista:
Prikl Biokhim Mikrobiol
Ano de publicação:
2011
Tipo de documento:
Article