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An ear punch model for studying the effect of radiation on wound healing.
Deoliveira, Divino; Jiao, Yiqun; Ross, Joel R; Corbin, Kayla; Xiao, Qizhen; Toncheva, Greta; Anderson-Evans, Colin; Yoshizumi, Terry T; Chen, Benny J; Chao, Nelson J.
Afiliação
  • Deoliveira D; Departments of Medicine, Duke University Medical Center, Durham, North Carolina, USA.
Int J Radiat Biol ; 87(8): 869-77, 2011 Aug.
Article em En | MEDLINE | ID: mdl-21480768
PURPOSE: Radiation and wound combined injury represents a major clinical challenge because of the synergistic interactions that lead to higher morbidity and mortality than either insult would produce singly. The purpose of this study was to develop a mouse ear punch model to study the physiological mechanisms underlying radiation effects on healing wounds. MATERIALS AND METHODS: Surgical wounds were induced by a 2 mm surgical punch in the ear pinnae of MRL/MpJ mice. Photographs of the wounds were taken and the sizes of the ear punch wounds were quantified by image analysis. Local radiation to the ear was delivered by orthovoltage X-ray irradiator using a specially constructed jig that shields the other parts of body. RESULTS: Using this model, we demonstrated that local radiation to the wound area significantly delayed the healing of ear punch wounds in a dose-dependent fashion. The addition of sublethal whole body irradiation (7 Gy) further delayed the healing of ear punch wounds. These results were replicated in C57BL/6 mice; however, wound healing in MRL/MpJ mice was accelerated. CONCLUSIONS: These data indicate that the mouse ear punch model is a valuable model to study radiation and wound combined injury.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cicatrização / Ferimentos Penetrantes / Modelos Animais de Doenças / Orelha Limite: Animals Idioma: En Revista: Int J Radiat Biol Assunto da revista: RADIOLOGIA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cicatrização / Ferimentos Penetrantes / Modelos Animais de Doenças / Orelha Limite: Animals Idioma: En Revista: Int J Radiat Biol Assunto da revista: RADIOLOGIA Ano de publicação: 2011 Tipo de documento: Article País de afiliação: Estados Unidos