Lhcb2 gene expression analysis in two ecotypes of Sedum alfredii subjected to Zn/Cd treatments with functional analysis of SaLhcb2 isolated from a Zn/Cd hyperaccumulator.
Biotechnol Lett
; 33(9): 1865-71, 2011 Sep.
Article
em En
| MEDLINE
| ID: mdl-21516315
ABSTRACT
The Lhcb2 gene from hyperaccumulator Sedum alfredii was up-regulated more than three-fold while the non-hyperaccumulator accumulated one or two-fold higher amount of the mRNA than control plants under different concentrations of Cd(2+) for 24 h. Lhcb2 expression was up-regulated more than five-fold in a non-hyperaccumulator S. alfredii when exposed to 2 µM Cd(2+) or 50 µM Zn(2+) for 8 d and the hyperaccumulator had over two-fold more mRNA abundance than the control plants. Over-expression of SaLhcb2 increased the shoot biomass by 14-41% and the root biomass by 21-57% without Cd(2+) treatment. Four transgenic tobacco lines (L5, L7, L10 and L11) possessed higher shoot biomass than WT plants with Cd(2+). Four transgenic lines (L7, L8, L10 and L11) accumulated 6-35% higher Cd(2+) amounts in shoots than the wild type plants.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Zinco
/
Cádmio
/
Regulação da Expressão Gênica de Plantas
/
Sedum
/
Proteínas de Ligação à Clorofila
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
China