Prediction of prognosis after resection in human hepatomas by nuclear-DNA analysis.

ABSTRACT

Nuclear DNA analysis with flow cytometry was performed in 81 patients with hepatocellular carcinoma. Thirty-eight cases (46.9%) were diploid type and forty-three cases (53.1%) aneuploid type. Microscopical portal vein invasion of the tumor was more frequent in the aneuploid group (p<0.05). Significantly higher frequency of intrahepatic metastasis was found in the aneuploid group (p<0.05). There was no correlation between the tumor nucleus DNA ploidy pattern and the recurrence rate, 23 (60.5%) of diploid cases and 28 (65.1%) of aneuploid cases, respectively. Disseminated recurrence was more frequent in the aneuploid group than in the diploid group (p<0.10). Significantly better survival rates were observed in the diploid group which had microscopical portal invasion. Moreover, the diploid group which had no capsular formation and intrahepatic metastasis showed significantly longer survival terms.