Functional analysis of the HS185 regulatory element in the rice HSP70 promoter.
Mol Biol Rep
; 39(2): 1649-57, 2012 Feb.
Article
em En
| MEDLINE
| ID: mdl-21633891
ABSTRACT
A series of HSP70 promoter deletion constructs was established. Analysis of beta-glucuronidase activities from the promoter deletion constructs in transient expression assays identified a cis-element, located from -493 to -308 bp upstream of the ATG start site. This element was designated as HS185 and has a crucial role in HSP70 promoter activity. HS185 has some characteristics of a miniature inverted-repeat transposable element (MITE), such as terminal inverted repeats (TIRs) (GGTCCCACA) and a putative target site duplication. There are 362 copies of homologous sequences of HS185 in the rice genome, which are preferentially distributed to non-coding regions. Based on these sequence features, we propose that HS185 is an uncharacterized rice MITE, possibly derived from the rice transposon Mutator-like element VIII family. Further transient expression assays showed that HS185 inhibited the enhancer activity of the cauliflower mosaic virus 35S promoter. These results demonstrate that not only is HS185 necessary for HSP70 promoter activity, but it also has a functional role as an insulator. This study explored new regulatory functions of non-coding repeat sequences in rice.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oryza
/
Elementos de DNA Transponíveis
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Regulação Viral da Expressão Gênica
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Proteínas de Choque Térmico HSP70
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Elementos Reguladores de Transcrição
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Mol Biol Rep
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China