[Study of galic acid extracted from the leaves of Phyllanthus emblica on apoptotic mechanism of human hepatocellular carcinoma cells BEL-7404].
Zhong Yao Cai
; 34(2): 246-9, 2011 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-21823485
ABSTRACT
OBJECTIVE:
To explore the mechanism of apoptosis of human hepatocellular carcinoma cells BEL-7404 induced by Galic acid extracted from leaves of Phyllanthus emblica.METHODS:
PI/Hoechst33342 double staining method was utilized to observe the influence on cell life cycle. The expressions of Bax and Bcl-2 protein were determined by fluorescence immunostaining and Western blot.RESULTS:
The results from PI/Hoechst33342 double staining method indicated that the percentage of cells G2/M phases increased after treated for 72 h, the phenomenon of blockage appeared and cell death was induced. Fluorescence immunostaining showed that the expression of Bax was up-regulated, and the expression of Bcl-2 was down-regulated. Western blot also showed that the the expression of Bax was up-regulated.CONCLUSION:
The mechanism of human hepatoma BEL-7404 cells apoptosis induced by Galic acid from leaves of Phyllanthus emblica may be blocking G2/M period in cell life cycle, up-regulating the expression of Bax and down-regulating the expression of Bcl-2, that can decrease membrane potential of mitochondria,and triggered the caspases of activation of cascade and induced cell death.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Phyllanthus emblica
/
Ácido Gálico
/
Neoplasias Hepáticas
/
Antineoplásicos Fitogênicos
Limite:
Humans
Idioma:
Zh
Revista:
Zhong Yao Cai
Assunto da revista:
TERAPIAS COMPLEMENTARES
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
China