Fungal recognition is mediated by the association of dectin-1 and galectin-3 in macrophages.
Proc Natl Acad Sci U S A
; 108(34): 14270-5, 2011 Aug 23.
Article
em En
| MEDLINE
| ID: mdl-21825168
ABSTRACT
Dectin-1, the major ß-glucan receptor in leukocytes, triggers an effective immune response upon fungal recognition. Here we use sortase-mediated transpeptidation, a technique that allows placement of a variety of probes on a polypeptide backbone, to monitor the behavior of labeled functional dectin-1 in live cells with and without fungal challenge. Installation of probes on dectin-1 by sortagging permitted highly specific visualization of functional protein on the cell surface and its subsequent internalization upon ligand presentation. Retrieval of sortagged dectin-1 expressed in macrophages uncovered a unique interaction between dectin-1 and galectin-3 that functions in the proinflammatory response of macrophages to pathogenic fungi. When macrophages expressing dectin-1 are exposed to Candida albicans mutants with increased exposure of ß-glucan, the loss of galectin-3 dramatically accentuates the failure to trigger an appropriate TNF-α response.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Candida albicans
/
Galectina 3
/
Macrófagos
/
Proteínas de Membrana
/
Proteínas do Tecido Nervoso
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos