Lysosomal membrane permeabilization is involved in curcumin-induced apoptosis of A549 lung carcinoma cells.
Mol Cell Biochem
; 359(1-2): 389-98, 2012 Jan.
Article
em En
| MEDLINE
| ID: mdl-21874542
ABSTRACT
We previously reported that curcumin inhibited lung cancer A549 cells growth and promoted cell apoptosis in vitro. In this study, we further examined the apoptosis-related parameters, including lysosomal damage and cathepsin activation, in A549 cells exposed to curcumin. We found that curcumin caused lysosomal membrane permeabilization (LMP) and cytosolic relocation of cathepsin B (cath B) and cathepsin D (cath D). However, only Z-FA-fmk (a cath B inhibitor) but not pepstatin A (a cath D inhibitor) inhibited curcumin-induced cell apoptosis, mitochondrial membrane potential loss, and cytochrome c release. The antioxidant N-acetylcysteine and glutathione attenuated LMP, suggesting that lysosomal destabilization was dependent on the elevation of reactive oxygen species and which precedes mitochondrial dysfunction. These findings indicated a novel pathway for curcumin regulation of ROS-lysosomal-mitochondrial pathway and provided the key mechanism of regulation of LMP in cell apoptosis, which may be exploited for cancer treatment.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoptose
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Curcumina
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Membranas Intracelulares
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Neoplasias Pulmonares
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Lisossomos
Limite:
Humans
Idioma:
En
Revista:
Mol Cell Biochem
Ano de publicação:
2012
Tipo de documento:
Article