Online monitoring of water toxicity by use of bioluminescent reporter bacterial biochips.
Environ Sci Technol
; 45(19): 8536-44, 2011 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-21875062
ABSTRACT
We describe a flow-through biosensor for online continuous water toxicity monitoring. At the heart of the device are disposable modular biochips incorporating agar-immobilized bioluminescent recombinant reporter bacteria, the responses of which are probed by single-photon avalanche diode detectors. To demonstrate the biosensor capabilities, we equipped it with biochips harboring both inducible and constitutive reporter strains and exposed it to a continuous water flow for up to 10 days. During these periods we challenged the biosensor with 2-h pulses of water spiked with model compounds representing different classes of potential water pollutants, as well as with a sample of industrial wastewater. The biosensor reporter panel detected all simulated contamination events within 0.5-2.5 h, and its response was indicative of the nature of the contaminating chemicals. We believe that a biosensor of the proposed design can be integrated into future water safety and security networks, as part of an early warning system against accidental or intentional water pollution by toxic chemicals.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Poluentes Químicos da Água
/
Sistemas On-Line
/
Técnicas Biossensoriais
/
Monitoramento Ambiental
/
Análise em Microsséries
/
Medições Luminescentes
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Environ Sci Technol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Israel