[Effects of PRL-3 siRNA on the migration of endometriotic stromal cells].
Zhonghua Yi Xue Za Zhi
; 91(23): 1613-6, 2011 Jun 21.
Article
em Zh
| MEDLINE
| ID: mdl-21914394
ABSTRACT
OBJECTIVE:
To evaluate the effects of PRL-3 siRNA (small interfering RNA) on the migration of endometriotic stromal cells.METHODS:
Primary endometriotic stromal cells were cultured in vitro. Then PRL-3 (phosphatase of regenerating liver-3) siRNA was transfected to silence the PRL-3 gene. And the PRL-3 protein expression was analyzed by Western blot. The changes of cell migration were detected by cell pseudopod formation, scratch test and transwell cell migration test.RESULTS:
The expression of PRL-3 protein significantly decreased in the experimental group versus two other control groups (P < 0.05). The formation of cell pseudopod was much less in experimental group than that in control groups. Within the same time, the number of migration cells was 0.87 ± 0.21 in experimental group. It was less than 1.75 ± 0.28 in blank control group and 1.63 ± 0.39 in negative control group (P < 0.05).CONCLUSION:
PRL-3 siRNA can down-regulate the PRL-3 gene and decrease the migratory capacity of endometriotic stromal cells. And PRL-3 may be a promising target in the therapeutics of endometriosis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Tirosina Fosfatases
/
Células Estromais
/
RNA Interferente Pequeno
/
Endométrio
/
Proteínas de Neoplasias
Limite:
Adult
/
Female
/
Humans
/
Middle aged
Idioma:
Zh
Revista:
Zhonghua Yi Xue Za Zhi
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
China