Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube.
Biotechnol Lett
; 34(1): 67-73, 2012 Jan.
Article
em En
| MEDLINE
| ID: mdl-21952914
ABSTRACT
Virus particles are used in vaccination, drug delivery, and material sciences. Here we devised a system where the RNA virus encephalomyocarditis virus (EMCV) is synthesized from DNA templates in vitro. When a plasmid or a PCR product harboring the full-length cDNA of EMCV in the T7 promoter/terminator unit was incubated in a HeLa cell extract supplemented with T7 RNA polymerase, EMCV was produced within 4 h at an efficiency of over 10-fold compared with the system programmed with the EMCV RNA. The EMCV RNA transcribed by the virally encoded RNA-dependent RNA polymerase was predominantly incorporated into the EMCV particle even in the presence of a larger amount of the EMCV RNA transcribed by T7 RNA polymerase from the plasmid.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
RNA Viral
/
Montagem de Vírus
/
Vírus da Encefalomiocardite
Limite:
Humans
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Japão