Effect of Lactobacillus plantarum and L-arginine against endotoxin-induced liver injury in a rat model.
Life Sci
; 89(23-24): 847-53, 2011 Dec 05.
Article
em En
| MEDLINE
| ID: mdl-21958471
ABSTRACT
AIMS:
Use of probiotics, alone or as adjunct to other established therapies, has been reported to have potential benefits. Recently, we have reported protective potential of probiotic against Salmonella-induced liver injury. However, co-supplementation with prebiotics did not result in meaningful synergism at systemic level. Owing to the action of probiotics at the mucosal level and of arginine at systemic level, the present study was designed to evaluate the effect of Lactobacillus plantarum alone or in conjunction with arginine to combat endotoxin-mediated liver injury in rats. MAINMETHODS:
Bacterial endotoxin/lipopolysaccharide (LPS) was injected intraperitoneally and animals were sacrificed 8h post-challenge. Efficacy of L. plantarum alone or in conjunction with l-arginine was determined on the basis of enzyme markers, histology, levels of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) in addition to identification of amino acids by paper chromatography. KEYFINDINGS:
Prior supplementation of LPS-challenged rats with L. plantarum (10(10)CFU per rat given orally for 10 days) demonstrated decreased levels of liver enzymes, NO and TNF-α. Interestingly, complementing Lactobacillus with arginine revealed a synergistic decrease not only in the liver markers but also in NO and TNF-α along with increased intensity of ornithine and methionine. Histological evidence also confirmed the protective efficacy of probiotic in conjunction with l-arginine.SIGNIFICANCE:
Presence of ornithine and methionine in the probiotic-arginine co-supplemented group suggests involvement of arginase-induced synthesis of polyamines. This study highlights that L. plantarum may direct l-arginine metabolism towards polyamine synthesis thereby exhibiting synergistic effect against liver injury.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arginina
/
Lipopolissacarídeos
/
Probióticos
/
Lactobacillus plantarum
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Doença Hepática Induzida por Substâncias e Drogas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Life Sci
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Índia