Design and synthesis of affinity ligands and relation of their structure with adsorption of proteins.
J Sep Sci
; 34(22): 3145-50, 2011 Nov.
Article
em En
| MEDLINE
| ID: mdl-22015803
ABSTRACT
Affinity chromatography has played an increasingly important role both in the pharmaceutical industry and academic research. In the present study, we report our preliminary investigation on the relationship between the affinity ligand structure and its adsorption to multi-protein samples. The structure of the ligands, including the size of the ring (cyclic group) and the length of the chain (linear group), has a great impact on the adsorption of ligands to proteins. Meanwhile, the functional groups that the ligands carry are also closely related to the adsorption of ligands to proteins. This research provides good guidance for the design and synthesis of affinity materials in affinity chromatography. It is also useful to other protein-ligand interaction-related research.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Resinas Sintéticas
/
Proteínas
/
Cromatografia de Afinidade
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2011
Tipo de documento:
Article