[Applications of MALDI-TOF-MS in clinical microbiology laboratory]. / Utilisation en routine du MALDI-TOF-MS pour l'identification des pathogènes en microbiologie médicale.
Med Sci (Paris)
; 27(10): 882-8, 2011 Oct.
Article
em Fr
| MEDLINE
| ID: mdl-22027426
ABSTRACT
For twenty years, mass spectrometry (MS) has emerged as a particularly powerful tool for analysis and characterization of proteins in research. It is only recently that this technology, especially MALDI-TOF-MS (Matrix Assisted Laser Desorption Ionization Time-Of-Flight) has entered the field of routine microbiology. This method has proven to be reliable and safe for the identification of bacteria, yeasts, filamentous fungi and dermatophytes. MALDI-TOF-MS is a rapid, precise and cost-effective method for identification, compared to conventional phenotypic techniques or molecular biology. Its ability to analyse whole microorganisms with few sample preparation has greatly reduced the time to identification (1-2 min). Furthermore, this technology can be used to identify bacteria directly from clinical samples as blood culture bottles or urines. Future applications will be developed in order to provide direct information concerning virulence or resistance protein markers.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Microbiológicas
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Fr
Revista:
Med Sci (Paris)
Assunto da revista:
MEDICINA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
França