The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4(+) cells.
Virology
; 423(2): 175-86, 2012 Feb 20.
Article
em En
| MEDLINE
| ID: mdl-22209231
ABSTRACT
It is generally believed that during the sexual transmission of HIV-1, the glycan-specific DC-SIGN receptor binds the virus and mediates its transfer to CD4(+) cells. The lectins griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN) inhibit HIV-1 infection by binding to mannose-rich glycans on gp120. We measured the ability of these lectins to inhibit both the HIV-1 binding to DC-SIGN and the DC-SIGN-mediated HIV-1 infection of CD4(+) cells. While GRFT, CV-N and SVN were moderately inhibitory to DC-SIGN binding, they potently inhibited DC-SIGN-transfer of HIV-1. The introduction of the 234 glycosylation site abolished HIV-1 sensitivity to lectin inhibition of binding to DC-SIGN and virus transfer to susceptible cells. However, the addition of the 295 glycosylation site increased the inhibition of transfer. Our data suggest that GRFT, CV-N and SVN can block two important stages of the sexual transmission of HIV-1, DC-SIGN binding and transfer, supporting their further development as microbicides.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Linfócitos T CD4-Positivos
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Proteínas de Transporte
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Moléculas de Adesão Celular
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Regulação para Baixo
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Infecções por HIV
/
HIV-1
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Receptores de Superfície Celular
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Proteínas de Algas
/
Lectinas Tipo C
Limite:
Humans
Idioma:
En
Revista:
Virology
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
África do Sul