Determination of folic acid in milk, milk powder and energy drink by an indirect immunoassay.
J Sci Food Agric
; 92(11): 2297-304, 2012 Aug 30.
Article
em En
| MEDLINE
| ID: mdl-22378547
ABSTRACT
BACKGROUND:
Folic acid (FA) is essential for healthy people (reference daily intake 400 µg day⻹) and pregnant women (600 µg day⻹). Insufficient intake of FA will increase the risk of neural tube defects in newborns. In this study an indirect enzyme-linked immunosorbent assay was developed for rapid and convenient detection of FA in vitamin-fortified foods.RESULTS:
A carbodiimide-modified active ester method was used to synthesise the immunogen (FA-bovine serum albumin (BSA) conjugate) to raise polyclonal antibodies for FA. The coupling ratio of FA with BSA was determined to be 141 (molar ratio). The detection limit of the immunoassay was 3.0 ng mL⻹ in buffer, 3.52 ng mL⻹ in energy drink, 11.91 ng mL⻹ in milk and 16.50 ng mL⻹ in milk powder. Intra- and inter-assay variability ranged from 6.6 to 15.1%. Analytical recoveries of FA-spiked samples were 88.3-108.9%.CONCLUSION:
The immunoassay developed in this study can be used as a simple, rapid and accurate method for fast semi-quantitative and quantitative on-site analysis of FA in food products.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bebidas
/
Alimentos Fortificados
/
Inspeção de Alimentos
/
Leite
/
Alimentos em Conserva
/
Ácido Fólico
Limite:
Animals
Idioma:
En
Revista:
J Sci Food Agric
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China