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VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation.
Matthijnssens, Jelle; Otto, Peter H; Ciarlet, Max; Desselberger, Ulrich; Van Ranst, Marc; Johne, Reimar.
Afiliação
  • Matthijnssens J; Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Belgium. jelle.matthijnssens@uz.kuleuven.ac.be
Arch Virol ; 157(6): 1177-82, 2012 Jun.
Article em En | MEDLINE | ID: mdl-22430951
Indirect immunofluorescence techniques targeting the rotavirus (RV) protein VP6 are used to differentiate RV species. The ICTV recognizes RV species A to E and two tentative species, F and G. A potential new RV species, ADRV-N, has been described. Phylogenetic trees and pairwise identity frequency graphs were constructed with more than 400 available VP6 sequences and seven newly determined VP6 sequences of RVD strains. All RV species were separated into distinct phylogenetic clusters. An amino acid sequence cutoff value of 53% firmly permitted differentiation of RV species, and ADRV-N was tentatively assigned to a novel RV species H (RVH).
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Aves Domésticas / Infecções por Rotavirus / Rotavirus / Proteínas do Capsídeo / Antígenos Virais Limite: Animals Idioma: En Revista: Arch Virol Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Bélgica

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Aves Domésticas / Infecções por Rotavirus / Rotavirus / Proteínas do Capsídeo / Antígenos Virais Limite: Animals Idioma: En Revista: Arch Virol Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Bélgica