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Validity of triple- and dual-tracer techniques to estimate glucose appearance.
Haidar, A; Elleri, D; Allen, J M; Harris, J; Kumareswaran, K; Nodale, M; Acerini, C L; Wilinska, M E; Jackson, N; Umpleby, A M; Evans, M L; Dunger, D B; Hovorka, R.
Afiliação
  • Haidar A; Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, United Kingdom.
Am J Physiol Endocrinol Metab ; 302(12): E1493-501, 2012 Jun 15.
Article em En | MEDLINE | ID: mdl-22454288
ABSTRACT
The triple-tracer (TT) dilution technique has been proposed to be the gold standard method to measure postprandial glucose appearance. However, validation against an independent standard has been missing. We addressed this issue and also validated the simpler dual-tracer (DT) technique. Sixteen young subjects with type 1 diabetes (age 19.5 ± 3.8 yr, BMI 23.4 ± 1.5 kg/m(2), HbA(1c) 8.7 ± 1.7%, diabetes duration 9.0 ± 6.9 yr, total daily insulin 0.9 ± 0.2 U·kg(-1)·day(-1), mean ± SD) received a variable intravenous 20% dextrose infusion enriched with [U-(13)C]glucose over 8 h to achieve postprandial-resembling glucose excursions while intravenous insulin was administered to achieve postprandial-resembling levels of plasma insulin. Primed [6,6-(2)H(2)]glucose was infused in a manner that mimicked the expected endogenous glucose production and [U-(13)C; 1,2,3,4,5,6,6-(2)H(7)]glucose was infused in a manner that mimicked the expected glucose appearance from a standard meal. Plasma glucose enrichment was measured by gas chromatography-mass spectrometry. The intravenous dextrose infusion served as an independent standard and was reconstructed using the TT and DT techniques with the two-compartment Radziuk/Mari model and an advanced stochastic computational method. The difference between the infused and reconstructed dextrose profile was similar for the two methods (root mean square error 6.6 ± 1.9 vs. 8.0 ± 3.5 µmol·kg(-1)·min(-1), TT vs. DT, P = NS, paired t-test). The TT technique was more accurate in recovering the overall dextrose infusion (100 ± 9 and 92 ± 12%; P = 0.02). The root mean square error associated with the mean dextrose infusion profile was 2.5 and 3.3 µmol·kg(-1)·min(-1) for the TT and DT techniques, respectively. We conclude that the TT and DT techniques combined with the advanced computational method can measure accurately exogenous glucose appearance. The TT technique tends to outperform slightly the DT technique, but the latter benefits from reduced experimental and computational complexity.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traçadores Radioativos / Técnica de Diluição de Radioisótopos / Glucose Tipo de estudo: Prognostic_studies Limite: Adolescent / Adult / Female / Humans / Male Idioma: En Revista: Am J Physiol Endocrinol Metab Assunto da revista: ENDOCRINOLOGIA / FISIOLOGIA / METABOLISMO Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traçadores Radioativos / Técnica de Diluição de Radioisótopos / Glucose Tipo de estudo: Prognostic_studies Limite: Adolescent / Adult / Female / Humans / Male Idioma: En Revista: Am J Physiol Endocrinol Metab Assunto da revista: ENDOCRINOLOGIA / FISIOLOGIA / METABOLISMO Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Reino Unido