Development and validation of an intrinsic landmark-based gating protocol applicable for functional and molecular ultrasound imaging.
Eur Radiol
; 22(8): 1789-96, 2012 Aug.
Article
em En
| MEDLINE
| ID: mdl-22460059
ABSTRACT
OBJECTIVES:
To implement a retrospective intrinsic landmark-based (ILB) gating protocol for contrast-enhanced ultrasound (CEUS) and to compare its efficiency to non-gated, manually gated and extrinsically gated CEUS.METHODS:
CEUS of the liver was performed in healthy mice (n = 5) and in NEMO knockout mice with dysplastic livers (n = 5). In healthy animals, first-pass kinetics of non-specific microbubbles was recorded. Knockout mice were analysed regarding retention of VEGFR2-specific microbubbles. For retrospective gating, a landmark which showed respiratory movement was encircled as a region of interest (ROI). During inspiration, the signal intensity within the ROI altered, which served as gating signal. To evaluate the accuracy, non-gated, extrinsically gated and ILB-gated time-intensity curves were created. For each curve, descriptive parameters were calculated and compared to the gold standard (manual frame-by-frame gating).RESULTS:
No significant differences in the variation of ILB- and extrinsically gated time-intensity curves from the gold standard were observed. Non-gated data showed significantly higher variations. Also the variation of molecular ultrasound data was significantly lower for ILB-gated compared to non-gated data.CONCLUSION:
ILB gating is a robust and easy method to improve data accuracy in functional and molecular ultrasound liver imaging. This technique can presumably be translated to contrast-enhanced ultrasound examinations in humans. KEY POINTS ⢠Quantitative analysis of the uptake of contrast agents during ultrasound is complex. ⢠Intrinsic landmark-based gating (ILB) offers a simple implementable method for motion correction. ⢠Results using ILB-gating are comparable to extrinsic gating using external biomonitoring devices. ⢠Functional and molecular imaging of mobile organs will benefit from ILB gating.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ultrassonografia
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Eur Radiol
Assunto da revista:
RADIOLOGIA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Alemanha