Depletion of mitochondrial fission factor DRP1 causes increased apoptosis in human colon cancer cells.
Biochem Biophys Res Commun
; 421(1): 81-5, 2012 Apr 27.
Article
em En
| MEDLINE
| ID: mdl-22487795
ABSTRACT
Mitochondria play a critical role in regulation of apoptosis, a form of programmed cell death, by releasing apoptogenic factors including cytochrome c. Growing evidence suggests that dynamic changes in mitochondrial morphology are involved in cellular apoptotic response. However, whether DRP1-mediated mitochondrial fission is required for induction of apoptosis remains speculative. Here, we show that siRNA-mediated DRP1 knockdown promoted accumulation of elongated mitochondria in HCT116 and SW480 human colon cancer cells. Surprisingly, DRP1 down-regulation led to decreased proliferation and increased apoptosis of these cells. A higher rate of cytochrome c release and reductions in mitochondrial membrane potential were also revealed in DRP1-depleted cells. Taken together, our present findings suggest that mitochondrial fission factor DRP1 inhibits colon cancer cell apoptosis through the regulation of cytochrome c release and mitochondrial membrane integrity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Neoplasias do Colo
/
Proteínas Mitocondriais
/
GTP Fosfo-Hidrolases
/
Proteínas de Membrana
/
Proteínas Associadas aos Microtúbulos
Tipo de estudo:
Etiology_studies
Limite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Japão