The double-helix microscope super-resolves extended biological structures by localizing single blinking molecules in three dimensions with nanoscale precision.
Appl Phys Lett
; 100(15): 153701-1537013, 2012 Apr 09.
Article
em En
| MEDLINE
| ID: mdl-22550359
ABSTRACT
The double-helix point spread function microscope encodes the axial (z) position information of single emitters in wide-field (x,y) images, thus enabling localization in three dimensions (3D) inside extended volumes. We experimentally determine the statistical localization precision σ of this approach using single emitters in a cell under typical background conditions, demonstrating σ < 20 nm laterally and <30 nm axially for N ≈ 1180 photons per localization. Combined with light-induced blinking of single-molecule labels, we present proof-of-concept imaging beyond the optical diffraction limit of microtubule network structures in fixed mammalian cells over a large axial range in three dimensions.
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01-internacional
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MEDLINE
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En
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Appl Phys Lett
Ano de publicação:
2012
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Article