The prototypic class Ia ribonucleotide reductase from Escherichia coli: still surprising after all these years.
Biochem Soc Trans
; 40(3): 523-30, 2012 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-22616862
ABSTRACT
RNRs (ribonucleotide reductases) are key players in nucleic acid metabolism, converting ribonucleotides into deoxyribonucleotides. As such, they maintain the intracellular balance of deoxyribonucleotides to ensure the fidelity of DNA replication and repair. The best-studied RNR is the class Ia enzyme from Escherichia coli, which employs two subunits to catalyse its radical-based reaction ß2 houses the diferric-tyrosyl radical cofactor, and α2 contains the active site. Recent applications of biophysical methods to the study of this RNR have revealed the importance of oligomeric state to overall enzyme activity and suggest that unprecedented subunit configurations are in play. Although it has been five decades since the isolation of nucleotide reductase activity in extracts of E. coli, this prototypical RNR continues to surprise us after all these years.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ribonucleotídeo Redutases
/
Escherichia coli
Idioma:
En
Revista:
Biochem Soc Trans
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos