High throughput single molecule tracking for analysis of rare populations and events.
Analyst
; 137(13): 2987-96, 2012 Jul 07.
Article
em En
| MEDLINE
| ID: mdl-22617120
ABSTRACT
High throughput single molecule tracking methods were developed to perform quantitative analyses of rare molecular populations. An optimization strategy for single molecule tracking at interfaces is described that allowed tracking of ~10(6) unique trajectories. These large statistical datasets were analyzed in order to identify and characterize distinct molecular populations based on their characteristic dynamic behavior (residence time or surface diffusion) and/or their spatial distribution. Cumulative (i.e. integrated) probability distributions were found to be several orders of magnitude more sensitive to rare populations than were raw probability distributions. Mapping using Accumulated Probe Trajectories (MAPT) was used to characterize molecular populations associated with rare surface heterogeneities. Importantly, large sample sizes were found to result in a dramatic enhancement in the ability to identify rare populations and to resolve their dynamic and spatial parameters.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Probabilidade
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Analyst
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos