Blood leukocytes from benznidazole-treated indeterminate chagas disease patients display an overall type-1-modulated cytokine profile upon short-term in vitro stimulation with Trypanosoma cruzi antigens.
BMC Infect Dis
; 12: 123, 2012 May 24.
Article
em En
| MEDLINE
| ID: mdl-22625224
ABSTRACT
BACKGROUND:
Benznidazole (Bz)-chemotherapy is recommended to prevent Chagas disease progression, despite its limited efficacy during chronic disease. However, the host mechanisms underlying these benefits still remain to be elucidated.METHODS:
In this study, we have used short-term whole blood cultures to describe the cytokine profile of Bz-treated Indeterminate Chagas disease patients-(INDt) as compared to untreated patients-(IND).RESULTS:
Our findings showed that IND presented increased levels of IL-10+neutrophils, IL-12⺠and IL-10⺠monocytes and IFN-γâºNK-cells. Moreover, IND showed slight increase of IL-4âºCD4âºT-cells and enhanced levels of IL-10âºCD8âºT-cells and B-cells. Additional analysis of cytokine Low and High producers also highlighted the presence of High cytokine producers within IND, including IL-10 from CD4⺠T-cells and IFN-γ from CD8⺠T-cells, as compared to NI. The Bz-treatment lead to an overall cytokine down-regulation in the innate and adaptive compartments, including low levels of IL-12⺠and IL-10⺠neutrophils and monocytes, IFN-γâºNK-cells, IL-12âº, TNF-αâº, IFN-γ⺠and IL-5âºCD4âºT-cells and IL-10âºB-cells, along with basal levels of cytokine-expressing CD8âºT-cells in INDt as compared to IND. The in vitro antigen stimulation shifted the cytokine profile toward a type 1-modulated profile, with increased levels of IL-12⺠and IL-10⺠monocytes, IFN-γ⺠and IL-4âºNK-cells along with TNF-α⺠and IFN-γâºCD8âºT-cells. Analysis of Low and High cytokine producers, upon in vitro antigen stimulation, further confirm these data.CONCLUSION:
Together, our findings showed that the Bz treatment of Indeterminate Chagas' disease patients shifts the cytokine patterns of peripheral blood monocytes, NK-cells and CD8⺠T-cells towards a long-lasting Type-1-modulated profile that could be important to the maintenance of a non-deleterious immunological microenvironment.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trypanosoma cruzi
/
Leucócitos Mononucleares
/
Citocinas
/
Doença de Chagas
/
Nitroimidazóis
/
Antiprotozoários
Tipo de estudo:
Observational_studies
/
Prevalence_studies
/
Risk_factors_studies
Limite:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
BMC Infect Dis
Assunto da revista:
DOENCAS TRANSMISSIVEIS
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Brasil