Halarchaeum salinum sp. nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt.
Int J Syst Evol Microbiol
; 63(Pt 3): 1138-1142, 2013 Mar.
Article
em En
| MEDLINE
| ID: mdl-22753525
ABSTRACT
Three halophilic archaeal strains, MH1-34-1(T), MH1-16-1 and MH1-224-5 were isolated from commercial salt samples produced from seawater in Indonesia, the Philippines and Japan, respectively. Cells of the three strains were pleomorphic and stained Gram-negative. Strain MH1-34-1(T) was orange-red pigmented, while MH1-16-1 and MH1-224-5 were pink-pigmented. Strain MH1-34-1(T) was able to grow at 12-30â% (w/v) NaCl (with optimum at 18â% NaCl, w/v) at pH 4.5-7.2 (optimum, pH 5.2-5.5) and at 15-45 °C (optimum, 42 °C). Strains MH1-16-1 and MH1-224-5 grew in slightly different ranges. These strains required at least 1 mM Mg(2+) for growth. The 16S rRNA gene sequences of strains MH1-34-1(T), MH1-16-1 and MH1-224-5 were almost identical (99.8-99.9â% similarities), and the closest relative was Halarchaeum acidiphilum MH-1-52-1(T) with 98.4â% similarities. The DNA G+C contents of MH1-34-1(T), MH1-16-1 and MH1-224-5 were 59.3, 60.8 and 61.0 mol%, respectively. The level of DNA-DNA relatedness amongst the three strains was 90-91â%, while that between each of the three strains and Halarchaeum acidiphilum MH1-52-1(T) was 51-55â%. Based on the phenotypic, genotypic and phylogenetic analyses, it is proposed that the isolates should represent a novel species of the genus Halarchaeum, for which the name Halarchaeum salinum sp. nov. is proposed. The type strain is MH1-34-1(T) (â=âJCM 16330(T)â=âCECT 7574(T)).
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Água do Mar
/
Cloreto de Sódio
/
Halobacteriaceae
País/Região como assunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Japão