Effective flow cytometric phenotyping of cells using minimal amounts of antibody.
Biotechniques
; 53(1): 57-60, 2012 Jul.
Article
em En
| MEDLINE
| ID: mdl-22780320
ABSTRACT
Here we introduce a modified antibody staining method that uses up to 80% less antibody for flow cytometry. We demonstrate this method for the detection of antigens expressed at high, moderate, or low levels in mouse and rat lymphocytes as well as mouse mammary epithelial cells. We obtained reproducibly accurate results for the detection of up to seven parameters for activation induced-proliferation, cell cycle analysis, and phenotyping of cell-surface and intracellular antigens.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos
/
Antígenos CD
/
Células Epiteliais
/
Citometria de Fluxo
Limite:
Animals
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos