Expression and significance of Kruppel-like factor 6 gene in osteosarcoma.
Int Orthop
; 36(10): 2107-11, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22855058
ABSTRACT
PURPOSE:
Osteosarcoma is primary malignant tumour of bone. Kruppel-like factor 6 (KLF6) is a tumor suppressor gene frequently inactivated in a number of human cancers and a ubiquitously expressed zinc-finger transcription factor. The present study aimed to first explore the relationship between the expression level of the KLF6 gene in osteosarcoma and the occurrence of bone tumours.METHODS:
KLF6 mRNA and protein expression levels in osteosarcoma and normal bone tissue were assayed by real-time quantitative PCR and immunohistochemistry. KLF6 mRNA and protein expression levels in osteosarcoma cells and normal osteoblasts were detected by semi-quantitative reverse transcription PCR and Western blotting, respectively.RESULTS:
Both the expression of KLF6 mRNA and protein in osteosarcoma cells and tissues were significantly lower than that in normal cells and tumour-adjacent tissues.CONCLUSIONS:
KLF6 is a putative tumor suppressor gene involved in osteosarcoma which can be used as a new therapeutic target and an important marker for early diagnosis and postoperative monitoring.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Ósseas
/
Osteossarcoma
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Proteínas Proto-Oncogênicas
/
Fatores de Transcrição Kruppel-Like
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Adolescent
/
Adult
/
Child
/
Female
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Int Orthop
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China