[Depot-specific expression of caveolin-1 in human adipose tissue and their relationship with obesity and insulin resistance].
Zhonghua Yi Xue Za Zhi
; 92(20): 1388-91, 2012 May 29.
Article
em Zh
| MEDLINE
| ID: mdl-22883195
ABSTRACT
OBJECTIVE:
To explore the depot-specific mRNA and protein expression of caveolin-1 (CAV-1) in human subcutaneous and omental adipose tissues and analyze their relationship with obesity and insulin resistance.METHODS:
A total of 41 subjects with normal glucose regulation were recruited. Among them, there were 29 cases with normal body mass index (BMI) and 12 cases with BMI ≥ 24 kg/m(2). All were scheduled to undergone selective abdominal operations. The levels of fasting insulin (FINS) were measured by chemiluminescence enzyme-linked immunosorbent assay (ELISA) kit. Fasting plasma glucose (FPG) was tested by glucose oxidase and home model insulin resistance index (HOMA-IR) calculated. Real-time polymerase chain reaction (RT-PCR) and Western blotting were employed to assess the relative mRNA and protein expression of caveolin-1 in subcutaneous and omental adipose tissues. And the mRNA and protein expression of caveolin-1 from different fat depots were compared and their correlations with BMI and HOMA-IR analyzed.RESULTS:
(1) FINS and HOMA-IR were significantly higher in the overweight and obesity group than those in the normal BMI group (FINS (8.82 ± 3.79) mU/L vs (6.43 ± 4.38) mU/L, P < 0.05, HOMA-1R 1.91 ± 0.85 vs 1.36 ± 0.72, P < 0.05). (2) The normal BMI group patients had the higher expression levels of caveolin-1 mRNA in omental adipose tissue than overweight counterparts (2.84 ± 0.86 vs 1.02 ± 0.36, P < 0.01). But the difference in subcutaneous adipose tissue was not significant (P > 0.05). (3) The caveolin-1 protein expression in omental adipose tissue of the normal BMI group was higher than that of overweight patients (1.68 ± 0.67 vs 0.73 ± 0.29, P < 0.05). And the difference between two groups was not significant (P > 0.05). (4)The expressions of caveolin-1 mRNA in omental adipose tissue were negatively correlated with BMI, waist circumstance, triglyceride and HOMA-IR (r = -0.441, -0.615, -0.539, -0.688, P < 0.05). No correlations were found between the expressions of caveolin-1 mRNA in subcutaneous adipose tissue with BMI, waist circumstance and HOMA-IR (P > 0.05).CONCLUSION:
Differential depot-specific expressions of caveolin-1 are present in human subcutaneous and omental adipose tissues. A low expression of caveolin-1 in omental adipose tissue may contribute to the pathogenesis of obesity and insulin resistance.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Resistência à Insulina
/
Tecido Adiposo
/
Caveolina 1
/
Obesidade
Tipo de estudo:
Observational_studies
Limite:
Adolescent
/
Adult
/
Female
/
Humans
/
Middle aged
Idioma:
Zh
Revista:
Zhonghua Yi Xue Za Zhi
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China