Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages.
Int J Nanomedicine
; 7: 4353-62, 2012.
Article
em En
| MEDLINE
| ID: mdl-22927755
ABSTRACT
Protein nanocages are self-organized complexes of oligomers whose three-dimensional architecture can been determined in detail. These structures possess nanoscale inner cavities into which a variety of molecules, including therapeutic or diagnostic agents, can be encapsulated. These properties yield these particles suitable for a new class of drug delivery carrier, or as a bioimaging reagent that might respond to biochemical signals in many different cellular processes. We report here the design, synthesis, and biological characterization of a hepatocyte-specific nanocage carrying small heat-shock protein. These nanoscale protein cages, with a targeting peptide composed of a preS1 derivative from the hepatitis B virus on their surfaces, were prepared by genetic engineering techniques. PreS1-carrying nanocages showed lower cytotoxicity and significantly higher specificity for human hepatocyte cell lines than other cell lines in vitro. These results suggested that small heat-shock protein-based nanocages present great potential for the development of effective targeted delivery of various agents to specific cells.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Precursores de Proteínas
/
Hepatócitos
/
Nanocápsulas
/
Antígenos de Superfície da Hepatite B
Limite:
Humans
Idioma:
En
Revista:
Int J Nanomedicine
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Japão