Co-transcriptional assembly of chemically modified RNA nanoparticles functionalized with siRNAs.
Nano Lett
; 12(10): 5192-5, 2012 Oct 10.
Article
em En
| MEDLINE
| ID: mdl-23016824
ABSTRACT
We report a generalized methodology for the one-pot production of chemically modified functional RNA nanoparticles during in vitro transcription with T7 RNA polymerase. The efficiency of incorporation of 2'-fluoro-dNTP in the transcripts by the wild type T7 RNA polymerase dramatically increases in the presence of manganese ions, resulting in a high-yield production of chemically modified RNA nanoparticles functionalized with siRNAs that are resistant to nucleases from human blood serum. Moreover, the unpurified transcription mixture can be used for functional ex vivo pilot experiments.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA
/
RNA Interferente Pequeno
/
Nanopartículas
Limite:
Humans
Idioma:
En
Revista:
Nano Lett
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos