Genomic profiling of a human leukemic monocytic cell-line (THP-1) exposed to alpha particle radiation.
ScientificWorldJournal
; 2012: 205038, 2012.
Article
em En
| MEDLINE
| ID: mdl-23097634
ABSTRACT
This study examined alpha (α-) particle radiation effects on global changes in gene expression in human leukemic monocytic cells (THP-1) for the purposes of mining for candidate biomarkers that could be used for the development of a biological assessment tool. THP-1 cells were exposed to α-particle radiation at a dose range of 0 to 1.5 Gy. Twenty-four hours and three days after exposure gene expression was monitored using microarray technology. A total of 16 genes were dose responsive and classified as early onset due to their expression 24 h after exposure. Forty-eight transcripts were dose responsive and classified as late-onset as they were expressed 72 h after exposure. Among these genes, 6 genes were time and dose responsive and validated further using alternate technology. These transcripts were upregulated and associated with biological processes related to immune function, organelle stability and cell signalling/communication. This panel of genes merits further validation to determine if they are strong candidate biomarkers indicative of α-particle exposure.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Monócitos
/
Regulação Neoplásica da Expressão Gênica
/
Leucemia Monocítica Aguda
/
Perfilação da Expressão Gênica
/
Partículas alfa
Limite:
Humans
/
Male
Idioma:
En
Revista:
ScientificWorldJournal
Assunto da revista:
MEDICINA
Ano de publicação:
2012
Tipo de documento:
Article