[Emergence of linezolid-resistant Enterococcus faecalis strains from two inpatients in a pediatric ward].
Kansenshogaku Zasshi
; 86(5): 555-62, 2012 Sep.
Article
em Ja
| MEDLINE
| ID: mdl-23198574
ABSTRACT
We report herein on the isolation of three linezolid-resistant Enterococcus faecalis strains in 2011 from two pediatric inpatients at Kitasato University Hospital, Japan. Three linezolid resistant strains were isolated from two patients who shared the same room of a pediatric inpatient ward. Two linezolid resistant strains were isolated from patient A who had been treated with a total of 17,600mg of linezolid during 60 days of hospitalization (strains 1 and 2). The linezolid resistant E. faecalis persisted through the time that the patient had been discharged from the hospital. Another linezolid resistant strain was isolated from patient B who had no history of linezolid administration. The resistant strain in patient B phased out spontaneously. The minimum inhibitory concentration of linezolid in these strains ranged from 8.0 to 16.0 microg/mL. PCR amplification of the chromosomal gene encoding domain V of the 23S rRNA and subsequent nucleotide sequencing revealed that all the strains had at least one G2576T mutation. The pulse-field-gel electrophoretograms of the DNA treated with the SmaI restriction enzyme showed an identical profile suggesting that they were derived from a single resistant strain. These results suggested that the resistant strain occurred in patient A and was transmitted to patient B within the inpatient ward.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Enterococcus faecalis
/
Oxazolidinonas
/
Acetamidas
/
Anti-Infecciosos
Limite:
Child
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Child, preschool
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Female
/
Humans
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Male
Idioma:
Ja
Revista:
Kansenshogaku Zasshi
Ano de publicação:
2012
Tipo de documento:
Article