Genotyping of rubella virus RNA in sera and dried blood spots collected during routine surveillance and in archival sera.
J Virol Methods
; 187(2): 284-7, 2013 Feb.
Article
em En
| MEDLINE
| ID: mdl-23201287
ABSTRACT
Information on the molecular epidemiology of rubella has been valuable in supporting efforts to control and eliminate rubella in several countries. The preferred samples for virus isolation or RNA detection, such as throat swabs, are often not available making it difficult to obtain a robust database of rubella virus sequences. A method for obtaining rubella virus genotypes from more commonly collected samples such as sera or dried blood spots using real-time RT-PCR to screen samples followed by nested set amplification is described. Rubella genotypes were obtained from dried blood spots and recent and archival sera collections. Eighteen percent of the RNAs extracted from the archival sera were real-time RT-PCR positive, and 44% of these RNAs were amplified successfully by nested RT-PCR and sequenced. Implementation of this technique could provide another tool to improve global rubella molecular surveillance.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Rubéola
/
Virologia
/
Sangue
/
RNA Viral
/
Reação em Cadeia da Polimerase
/
Reação em Cadeia da Polimerase em Tempo Real
Tipo de estudo:
Evaluation_studies
/
Screening_studies
Limite:
Humans
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos