Induction of sulfadiazine resistance in vitro in Toxoplasma gondii.
Exp Parasitol
; 133(2): 131-6, 2013 Feb.
Article
em En
| MEDLINE
| ID: mdl-23206954
ABSTRACT
We induced sulfadiazine resistance in two sulfadiazine sensitive strains of Toxoplasma gondii, RH (Type I) and ME-49 (Type II) in vitro by using drug pressure. At first, sulfadiazine susceptibility of the two sensitive strains and two naturally resistant strains of T. gondii was evaluated on Vero cells using an enzyme-linked immunosorbent assay (ELISA). The IC(50) values of sulfadiazine were 77 µg/mL for RH, 51 µg/mL for ME-49 and higher than 1000 µg/mL for the two natural resistant strains. Secondly, induced resistance of the strains by gradually increase sulfadiazine concentration was verified by this test, which resulted IC(50) values at higher than 1000 µg/mL. In conclusion we developed in vitro two sulfadiazine resistant strains called RH-R(SDZ) and ME-49-R(SDZ). These strains resistant to sulfadiazine would be useful to characterize resistance mechanisms to sulfadiazine.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sulfadiazina
/
Toxoplasma
/
Antiprotozoários
Limite:
Animals
Idioma:
En
Revista:
Exp Parasitol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
França