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Fine-mapping and molecular marker development for Pi56(t), a NBS-LRR gene conferring broad-spectrum resistance to Magnaporthe oryzae in rice.
Liu, Yan; Liu, Bin; Zhu, Xiaoyuan; Yang, Jianyuan; Bordeos, Alicia; Wang, Guoliang; Leach, Jan E; Leung, Hei.
Afiliação
  • Liu Y; International Rice Research Institute, Plant Breeding, Genetics and Biotechnology Division, DAPO Box 7777, Metro Manila, Philippines.
Theor Appl Genet ; 126(4): 985-98, 2013 Apr.
Article em En | MEDLINE | ID: mdl-23400829
ABSTRACT
The major quantitative trait locus qBR9.1 confers broad-spectrum resistance to rice blast, and was mapped to a ~69.1 kb region on chromosome 9 that was inherited from resistant variety Sanhuangzhan No 2 (SHZ-2). Within this region, only one predicted disease resistance gene with nucleotide binding site and leucine-rich repeat (NBS-LRR) domains was found. Specific markers corresponding to this gene cosegregated with blast resistance in F2 and F3 populations derived from crosses of susceptible variety Texianzhan 13 (TXZ-13) to SHZ-2 and the resistant backcross line BC-10. We tentatively designate the gene as Pi56(t). Sequence analysis revealed that Pi56(t) encodes an NBS-LRR protein composed of 743 amino acids. Pi56(t) was highly induced by blast infection in resistant lines SHZ-2 and BC-10. The corresponding allele of Pi56(t) in the susceptible line TXZ-13 encodes a protein with an NBS domain but without LRR domain, and it was not induced by Magnaporthe oryzae infection. Three new cosegregating gene-specific markers, CRG4-1, CRG4-2 and CRG4-3, were developed. In addition, we evaluated polymorphism of the gene-based markers among popular varieties from national breeding programs in Asia and Africa. The presence of the CRG4-2 SHZ-2 allele cosegregated with a blast-resistant phenotype in two BC2F1 families of SHZ-2 crossed to recurrent parents IR64-Sub1 and Swarna-Sub1. CRG4-1 and CRG4-3 showed clear polymorphism among 19 varieties, suggesting that they can be used in marker-assisted breeding to combine Pi56(t) with other target genes in breeding lines.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Oryza / Marcadores Genéticos / Magnaporthe / Locos de Características Quantitativas / Resistência à Doença Tipo de estudo: Prognostic_studies País/Região como assunto: Asia Idioma: En Revista: Theor Appl Genet Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Filipinas

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Oryza / Marcadores Genéticos / Magnaporthe / Locos de Características Quantitativas / Resistência à Doença Tipo de estudo: Prognostic_studies País/Região como assunto: Asia Idioma: En Revista: Theor Appl Genet Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Filipinas