Effect of cargo size and shape on the transport efficiency of the bacterial Tat translocase.
FEBS Lett
; 587(7): 912-6, 2013 Apr 02.
Article
em En
| MEDLINE
| ID: mdl-23422074
ABSTRACT
The Tat machinery translocates fully-folded and oligomeric substrates. The passage of large, bulky cargos across an ion-tight membrane suggests the need to match pore and cargo size, and therefore that Tat transport efficiency may depend on both cargo size and shape. A series of cargos of different sizes and shapes were generated using the natural Tat substrate pre-SufI as a base. Four (of 17) cargos transported with significant (>20% of wild-type) efficiencies. These results indicate that cargo size and shape significantly influence Tat transportability.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
/
Membrana Celular
/
Proteínas de Escherichia coli
/
Escherichia coli
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos