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Effect of calf starter feeding on gut microbial diversity and expression of genes involved in host immune responses and tight junctions in dairy calves during weaning transition.
Malmuthuge, Nilusha; Li, Meiju; Goonewardene, Laksiri A; Oba, Masahito; Guan, L Luo.
Afiliação
  • Malmuthuge N; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
J Dairy Sci ; 96(5): 3189-200, 2013 May.
Article em En | MEDLINE | ID: mdl-23498024
Calf starters are usually offered to dairy calves to facilitate the weaning process, however, the effect of solid feed consumption on gut health has not been well studied. This study aimed to investigate the effect of calf starter feeding on the gut bacterial community and mucosal immune functions in dairy calves during weaning transition. Mucosal tissue and digesta samples were collected from rumen, jejunum, ileum, cecum, and colon upon slaughtering of calves (n=8) after feeding the experimental diets [milk replacer (MR) or milk replacer + calf starter (MR+S)] for 6 wk. Expression of toll-like receptor (TLR) 10 was downregulated along the gut, whereas TLR2 in colon and TLR6 along the gut were upregulated in MR+S-fed calves compared with MR-fed calves. Ileal TLR9 and TLR10 showed higher expression compared with the other regions regardless of the diet. Peptidoglycan recognition protein 1 demonstrated a diet- and gut-regional dependent expression pattern, whereas ß-defensin did not. The diet and gut region also affected the expression of tight junction-regulating genes claudin 4 and occludin. Bacterial diversity tended to be different between the 2 diets, whereas the bacterial density was different among gut regions and sample type. The present study revealed that changes in bacterial diversity, expression of genes encoding host mucosal immune responses, and barrier functions were associated with the MR+S diet, and suggests that solid feed consumption may alter gut microbiome and host mucosal functions during weaning transition.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Desmame / Regulação da Expressão Gênica / Junções Íntimas / Trato Gastrointestinal / Imunidade / Ração Animal / Animais Lactentes Limite: Animals Idioma: En Revista: J Dairy Sci Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Canadá

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Desmame / Regulação da Expressão Gênica / Junções Íntimas / Trato Gastrointestinal / Imunidade / Ração Animal / Animais Lactentes Limite: Animals Idioma: En Revista: J Dairy Sci Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Canadá