Antigen identification using skin deficient in basement-membrane protein: a novel tool for the diagnosis of subepidermal immunobullous diseases.
Clin Exp Dermatol
; 38(3): 289-94; quiz 294, 2013 Apr.
Article
em En
| MEDLINE
| ID: mdl-23517360
ABSTRACT
Common unifying features of the subepidermal blistering diseases are the presence of tense blisters clinically and demonstration by immunofluorescence of linear deposition of immunoreactants along the dermoepidermal junction. Further characterization of subtype is possible by identification of the target antigen by immunoblotting. However, immunoblotting is time-consuming and may not be practical for routine use in the laboratory. In this report, we describe a simple technique to identify the target antigen by indirect immunofluorescence, using epidermolysis bullosa skin as substrate.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Membrana Basal
/
Epidermólise Bolhosa
/
Técnica Indireta de Fluorescência para Anticorpo
/
Proteínas de Membrana
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Clin Exp Dermatol
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Índia