Quantum dot imaging for HSP70 and HSF1 kinetics in SCC25 cells with or without leucine deprivation following heat shock.
Oncol Rep
; 29(6): 2255-60, 2013 Jun.
Article
em En
| MEDLINE
| ID: mdl-23546225
ABSTRACT
The aim of this study was to develop a quantum dot-based approach for heat shock protein 70 (HSP70) and heat shock factor 1 (HSF-1) kinetics following heat shock, and to discover approaches to thermotherapy based on disrupting the effect of activation of HSF-1 and the accumulation of HSP70 by leucine deprivation. SCC-25 cells cultured with limiting leucine or normal leucine were stressed at 42ËC for 30 min, and were cultured for 2, 4, 6, 8 and 10 h, respectively. The expression of HSP70 and HSF-1 was observed using confocal laser microscopy and semi-quantitative analysis was performed by Image-Pro Plus. At 6 h after heating, HSF-1 in cells cultured with normal leucine was activated and translocated from the cytosol to the nucleus, and the synthesis of HSP70 reached the maximum value and had a tendency to gather in the nucleus. However, in cells cultured with limiting leucine, HSF-1 activity decreased and accumulation of HSP70 was not found. Leucine deprivation results in the inactivation of HSF-1 leading to slight accumulation of HSP70 and no tendency to gather in the nucleus. Thus, HSF-1 may serve as a novel therapeutic target in the treatment of oral cancer.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Proteínas de Choque Térmico HSP70
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Resposta ao Choque Térmico
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Proteínas de Ligação a DNA
/
Leucina
Limite:
Humans
Idioma:
En
Revista:
Oncol Rep
Assunto da revista:
NEOPLASIAS
Ano de publicação:
2013
Tipo de documento:
Article