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Normal and tumour cervical cells respond differently to vaginal lactobacilli, independent of pH and lactate.
Motevaseli, Elahe; Shirzad, Mahdieh; Akrami, Seyed Mohammad; Mousavi, Azam-Sadat; Mirsalehian, Akbar; Modarressi, Mohammad Hossein.
Afiliação
  • Motevaseli E; Department of Medical Biotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran.
  • Shirzad M; Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
  • Akrami SM; Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
  • Mousavi AS; Department of Gynecology Oncology, Valli-e-Asr Hospital, Tehran University of Medical Sciences, Tehran, Iran.
  • Mirsalehian A; Department of Microbiology, Tehran University of Medical Sciences, Tehran, Iran.
  • Modarressi MH; Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran.
J Med Microbiol ; 62(Pt 7): 1065-1072, 2013 Jul.
Article em En | MEDLINE | ID: mdl-23618799
ABSTRACT
Cervical cancer is a human papilloma virus (HPV)-related cancer, but most HPV infections are transient or intermittent and resolve spontaneously. Thus, other factors, such as cervical microflora, which are dominated by lactobacilli, must be involved in invasive cervical carcinoma development after HPV infection. Previous studies have demonstrated that lactobacilli have antitumour effects, and it is possible that vaginal lactobacilli prevent cervical cancer. Here we examined the proliferative and apoptotic responses of normal and tumour cervical cells to common vaginal lactobacilli components by investigating human normal fibroblast-like cervical (normal cervical) and HeLa (cervical tumour) cell responses to Lactobacillus gasseri and Lactobacillus crispatus. The effects of different lactobacilli components, such as culture supernatants, cytoplasmic extracts, cell-wall extracts and live cells, were determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, trypan blue staining, lactate dehydrogenase assay and colorimetric caspase-3 activity assay. Changes in caspase-3 and human chorionic gonadotropin ß (hCGß) expression were analysed by quantitative RT-PCR. Tumour cell growth inhibition by culture supernatants was higher than that by pH- and lactate-adjusted controls. However, the effects of the supernatants on normal cells were similar to those of lactate-adjusted controls. Apoptosis was inhibited by supernatants, which was consistent with higher hCGß expression since hCG inhibits apoptosis. Our study demonstrated that common vaginal lactobacilli exert cytotoxic effects on cervical tumour cells, but not on normal cells, and that this cytotoxicity is independent of pH and lactate. Our results encourage further studies on the interaction between lactobacilli and cervical cells, and administration of common vaginal lactobacilli as probiotics.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vagina / Neoplasias do Colo do Útero / Ácido Láctico / Lactobacillus Limite: Female / Humans Idioma: En Revista: J Med Microbiol Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Irã

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vagina / Neoplasias do Colo do Útero / Ácido Láctico / Lactobacillus Limite: Female / Humans Idioma: En Revista: J Med Microbiol Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Irã