A perspective on mammalian upstream open reading frame function.
Int J Biochem Cell Biol
; 45(8): 1690-700, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23624144
ABSTRACT
Post-transcriptional control makes a major contribution to the overall regulation of gene expression pathway. Within the cytoplasm this is mediated by a combination of regulatory RNA motifs within the 5' and 3' untranslated regions of mRNAs and their interacting protein/RNA partners. One of the most common regulatory RNA elements in mammalian transcripts (present in approximately 40% of all mRNAs) are upstream open reading frames (uORFs). However, despite the prevalence of these RNA elements how they function is not well understood. In general, they act to repress translation of the physiological ORF under control conditions, and under certain pathophysiological stresses this repression can be alleviated. It is known that re-initiation following the translation of an uORF is utilised in some situations however there are numerous alternative mechanisms that control the synthesis of a protein whose mRNA contains uORFs. Moreover, the trans-acting factors that are also involved in this process are not well defined. In this review we summarise our current understanding of this area and highlight some common features of these RNA motifs that have been discovered to date.
Palavras-chave
4E-binding proteins; 4EBPs; AdoMetDCS; EMCV; Eif; IRES; NMD; Protein synthesis; RRL; SNP; THPO; Translational control; UTR; Upstream open reading frame; adenosyl-methionine decarboxylase; encephalomyocarditis virus; eukaryotic initiation factor; internal ribosome entry segment; nonsense mediated decay; rabbit reticulocyte lysate; single nucleotide polymorphism; thrombopoietin; uORF; untranslated region; upstream open reading frame
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fases de Leitura Aberta
/
Mamíferos
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Int J Biochem Cell Biol
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Reino Unido