Two-photon optical interrogation of individual dendritic spines with caged dopamine.
ACS Chem Neurosci
; 4(8): 1163-7, 2013 Aug 21.
Article
em En
| MEDLINE
| ID: mdl-23672485
ABSTRACT
We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for an optical imaging and manipulation of dendritic spines in living brain slices, demonstrating that spines can express functional dopamine receptors. This novel compound allows mapping of functional dopamine receptors in living brain tissue with exquisite spatial resolution.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rutênio
/
Dopamina
/
Receptores Dopaminérgicos
/
Córtex Pré-Frontal
/
Espinhas Dendríticas
Limite:
Animals
Idioma:
En
Revista:
ACS Chem Neurosci
Ano de publicação:
2013
Tipo de documento:
Article