Evaluation of the prostaglandin F synthase activity of human and bovine aldo-keto reductases: AKR1A1s complement AKR1B1s as potent PGF synthases.
Prostaglandins Other Lipid Mediat
; 106: 124-32, 2013 Oct.
Article
em En
| MEDLINE
| ID: mdl-23747692
ABSTRACT
AKR1B1 of the polyol pathway was identified as a prostaglandin F2α synthase (PGFS). Using a genomic approach we have identified in the endometrium five bovine and three human AKRs with putative PGFS activity and generated the corresponding recombinant enzymes. The PGFS activity of the recombinant proteins was evaluated using a novel assay based on in situ generation of the precursor of PG biosynthesis PGH2. PGF2α was measured by ELISA and the relative potencies of the different enzymes were compared. We identified AKR1A1 and confirmed AKR1B1 as the most potent PGFS expressing characteristic inhibition patterns in presence of methylglyoxal, ponalrestat and glucose.
Palavras-chave
AA; AKR; AKR1A1; AKR1B1; AKR1C; Aldose reductase; BLOTTO; COX; DD3; Endometrium; IL-1ß; PBS with Tween 20; PBS-T; PGD2; PGE2; PGF2α; PGF2α synthase; PGFS; PGG2; PGH2; PGs; aldo-keto reductases; arachidonic acid; cyclooxygenase; dihydrodiol dehydrogenase 3; fat-free dry milk in PBS-T; interleukine-1 beta; prostaglandin D2; prostaglandin E2; prostaglandin F2α; prostaglandin G2; prostaglandin H2; prostaglandins
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hidroxiprostaglandina Desidrogenases
/
Aldeído Redutase
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Prostaglandins Other Lipid Mediat
Assunto da revista:
ENDOCRINOLOGIA
Ano de publicação:
2013
Tipo de documento:
Article