Capturing intercellular sugar-mediated ligand-receptor recognitions via a simple yet highly biospecific interfacial system.
Sci Rep
; 3: 2293, 2013.
Article
em En
| MEDLINE
| ID: mdl-23892317
ABSTRACT
Intercellular ligand-receptor recognitions are crucial natural interactions that initiate a number of biological and pathological events. We present here the simple construction of a unique class of biomimetic interfaces based on a graphene-mediated self-assembly of glycosyl anthraquinones to a screen-printed electrode for the detection of transmembrane glycoprotein receptors expressed on a hepatoma cell line. We show that an electroactive interface confined with densely clustered galactosyl ligands is able to ingeniously recognize the asialoglycoprotein receptors on live Hep-G2 cells employing simple electrochemical techniques. The only facility used is a personal laptop in connection with a cheap and portable electrochemical workstation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carboidratos
/
Técnicas Biossensoriais
/
Receptores Citoplasmáticos e Nucleares
/
Metabolismo dos Carboidratos
/
Ligantes
Limite:
Humans
Idioma:
En
Revista:
Sci Rep
Ano de publicação:
2013
Tipo de documento:
Article