[Impact and potential mechanism of human α-defensin 1 on low-density lipoprotein oxidation ability of ECV304 cells].
Zhonghua Xin Xue Guan Bing Za Zhi
; 41(5): 406-10, 2013 May.
Article
em Zh
| MEDLINE
| ID: mdl-24021124
ABSTRACT
OBJECTIVE:
To explore the role and potential mechanism of human α-defensin 1 (HNP-1) on low-density lipoprotein (LDL) oxidation ability of human endothelial cells (EVC304).METHODS:
Post incubation with LDL for 3 h, the malondialdehyde (MDA) and protein carbonyl (PCO) were detected in untreated ECV304 (control) and in HNP-1 transfected ECV304 in the presence and absence of siRNA against HNP-1. Flow cytometry and fluorescence microscopy were used to detect the generation of oxygen free radical in the ECV304 which have been pretreated by LDL, LPS and HNP-1, respectively.RESULT:
Compared with control group, MDA level was significantly increased in HNP-1 transfected [(4.21 ± 0.03) vs. (3.15 ± 0.02) nmol/mg · pro] or in HNP-1 stimulated ECV304 cells [(14.49 ± 1.10) vs. (9.47 ± 1.18) nmol/mg · pro], which could be significantly downregulated by siRNA [(3.76 ± 0.48) vs. (4.54 ± 0.28) nmol/mg·pro, all P < 0.05]. PCO was also significantly increased in HNP-1 transfected ECV304 cells. The levels of free radical were significantly increased in HNP-1 transfected or HNP-1 stimulated ECV304 cells.CONCLUSION:
HNP-1 can enhance the LDL oxidation ability of human endothelial cells via promoting the generation of free radicals.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Alfa-Defensinas
/
Células Endoteliais
/
Lipoproteínas LDL
Limite:
Humans
Idioma:
Zh
Revista:
Zhonghua Xin Xue Guan Bing Za Zhi
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China