Noninvasive assessment of alveolar microvascular recruitment in conscious non-sedated rats.
Respir Physiol Neurobiol
; 190: 105-12, 2014 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-24100202
ABSTRACT
Recruitment of alveolar microvascular reserves, assessed from the relationship between pulmonary diffusing capacity (DLCO) and perfusion (QËc), is critical to the maintenance of arterial blood oxygenation. Leptin-resistant ZDF fatty diabetic (fa/fa) rats exhibit restricted cardiopulmonary physiology under anesthesia. To assess alveolar microvascular function in conscious, non-sedated, non-instrumented, and minimally restrained animals, we adapted a rebreathing technique to study fa/fa and control non-diabetic (+/+) rats (4-5 and 7-11mo old) at rest and during mild spontaneous activity. Measurements included O2 uptake, lung volume, QËc, DLCO, membrane diffusing capacity (DMCO), capillary blood volume (Vc) and septal tissue-blood volume. In older fa/fa than +/+ animals, DLCO and DMCO at a given QËc were lower; Vc was reduced in proportion to QËc. Results demonstrate the consequences of alveolar microangiopathy in the metabolic syndrome lung volume restriction, reduced QËc, and elevated membrane resistance to diffusion. At a given QËc, DLCO is lower in rats and guinea pigs than dogs or humans, consistent with limited alveolar microvascular reserves in small animals.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Alvéolos Pulmonares
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Capacidade de Difusão Pulmonar
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Circulação Pulmonar
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Estado de Consciência
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Respir Physiol Neurobiol
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Estados Unidos